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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be noticed in several tumors, which ends up in chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a essential function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants have been documented but specific nucleotide sequence details is not still obtainable.

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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide normally incorporates the epitope recognized with the antibody.

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This guidebook outlines more feasible optimization of mobile and immunoassay parameters to make sure the best possible final results are...

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This guidebook outlines more attainable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...

Antibodies certain to the blocking peptide no more bind on the epitope about the goal protein. By comparing the staining in the blocked antibody versus the antibody by itself, you can see which staining is precise.

Focus on Data Cyclin E belongs for the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...

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